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    Biocontrol of foodborne bacterial pathogens using immobilized bacteriophages.

    機譯:使用固定的噬菌體對食源性細菌病原體進行生物防治。

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    摘要

    The goal of the present research was to develop a simple technique to immobilize isolated lytic phages and explore the potential use of these immobilized phages to control certain foodborne pathogens in a real food system. More than one hundred phages were isolated from different environmental samples against different strains of four major foodborne pathogens; E. coli O157:H7, Salmonella, Listeria monocytogenes and Shigella. A turbidimetric method in high throughput format using the Bioscreen C was used to monitor phage lytic activity and determine the host range of the isolated phages. This enabled identification of isolated phages with similar characteristics and the selection of twelve good phage candidates for biocontrol purposes. These phages were characterized by TEM, restriction endonuclease pattern, one-step growth curve, BIM (bacteriphage insensitve mutant) development, cross infectivity and determination of their stability and infectivity under different conditions. A novel Shigella phage, &PHgr;SboM-AG3, was isolated and its genome sequenced. Its genome did not show any homology to any reported virulent or lysogenic genes and it was considered as a member of the "T4 superfamily". Phage cocktails made from the isolated phages were very effective for specific control of the four target pathogens in both broth media and contaminated food stored under different environmental conditions. The phages were immobilized using positively charged carrier substrates, which allowed specific 'binding of phages through their heads and leaving tail fibers free to interact with bacteria. These immobilized phages retained infectivity and immobilized Listeria and E. coli phage cocktails were able to control the growth of L. monocytogenes and E. coli O157:H7, respectively, in food under different temperatures and packaging conditions.
    機譯:本研究的目的是開發一種固定化分離的裂解噬菌體的簡單技術,并探索這些固定化噬菌體在實際食品系統中控制某些食源性病原體的潛在用途。從不同環境樣品中針對四種主要食源性病原體的不同菌株分離出一百多個噬菌體。大腸桿菌O157:H7,沙門氏菌,李斯特菌和志賀氏菌。使用Bioscreen C的高通量比濁法用于監測噬菌體的裂解活性并確定分離噬菌體的宿主范圍。這使得能夠鑒定具有相似特征的分離的噬菌體并選擇十二種良好的噬菌體候選物用于生物防治。這些噬菌體的特征在于TEM,限制性內切核酸酶模式,一步生長曲線,BIM(細菌不敏感突變體)發育,交叉感染性以及在不同條件下確定其穩定性和感染性。分離出新的志賀氏菌噬菌體&SgrM-AG3,并對其基因組進行測序。它的基因組與任何報道的有毒力或溶原性基因沒有任何同源性,被認為是“ T4超家族”的成員。由分離的噬菌體制成的噬菌體雞尾酒對于肉湯培養基和在不同環境條件下存儲的受污染食品中的四種目標病原體的特異性控制非常有效。使用帶正電的載體底物固定噬菌體,這可使噬菌體通過其頭部進行特定的“結合”,并使尾巴纖維自由與細菌相互作用。這些固定的噬菌體保留了傳染性,固定的李斯特菌和大腸桿菌噬菌體混合物能夠分別控制食品在不同溫度和包裝條件下單核細胞增生李斯特菌和大腸桿菌O157:H7的生長。

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    • 作者單位

      University of Guelph (Canada).;

    • 授予單位 University of Guelph (Canada).;
    • 學科 Agriculture Food Science and Technology.;Biology Microbiology.
    • 學位 Ph.D.
    • 年度 2010
    • 頁碼 284 p.
    • 總頁數 284
    • 原文格式 PDF
    • 正文語種 eng
    • 中圖分類
    • 關鍵詞

    • 入庫時間 2022-08-17 11:36:56

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